Organismal, genetic, and transcriptional variation in the deeply sequenced gut microbiomes of identical twins.

ABSTRACT: We deeply sampled the organismal, genetic, and transcriptional diversity in fecal samples collected from a monozygotic (MZ) twin pair and compared the results to 1,095 communities from the gut and other body habitats of related and unrelated individuals. Using a new scheme for noise reduction in pyrosequencing data, we estimated the total diversity of species-level bacterial phylotypes in the 1.2-1.5 million bacterial 16S rRNA reads obtained from each deeply sampled cotwin to be approximately 800 (35.9%, 49.1% detected in both). A combined 1.1 million read 16S rRNA dataset representing 281 shallowly sequenced fecal samples from 54 twin pairs and their mothers contained an estimated 4,018 species-level phylotypes, with each sample having a unique species assemblage (53.4 +/- 0.6% and 50.3 +

SUBMITTER: Turnbaugh PJ 

PROVIDER: S-EPMC2867707 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature