Globin-like proteins in Caenorhabditis elegans: in vivo localization, ligand binding and structural properties.

ABSTRACT:

Background

The genome of the nematode Caenorhabditis elegans contains more than 30 putative globin genes that all are transcribed. Although their translated amino acid sequences fit the globin fold, a variety of amino-acid substitutions and extensions generate a wide structural diversity among the putative globins. No information is available on the physicochemical properties and the in vivo expression.

Results

We expressed the globins in a bacterial system, characterized the purified proteins by optical and resonance Raman spectroscopy, measured the kinetics and equilibria of O2 binding and determined the crystal structure of GLB-1* (CysGH2 --> Ser mutant). Furthermore, we studied the expression patterns of glb-1 (ZK637.13) and glb-26 (T22C1.2) in the worms using green fluo

SUBMITTER: Geuens E 

PROVIDER: S-EPMC2867796 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature