Proteome analysis of human Wharton's jelly cells during in vitro expansion.

ABSTRACT:

Background

The human umbilical cord contains mucoid connective tissue and fibroblast-like cells. These cells named Wharton's jelly cells, (WJCs) display properties similar to mesenchymal stem cells therefore representing a rich source of primitive cells to be potentially used in regenerative medicine.

Results

To better understand their self-renewal and potential in vitro expansion capacity, a reference 2D map was constructed as a proteomic data set. 158 unique proteins were identified. More than 30% of these proteins belong to cytoskeleton compartment. We also found that several proteins including Shootin1, Adenylate kinase 5 isoenzyme and Plasminogen activator-inhibitor 2 are no longer expressed after the 2nd passage of in vitro replication. This indicates that the prolifer

SUBMITTER: Angelucci S 

PROVIDER: S-EPMC2867805 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature