The response to unfolded protein is involved in osmotolerance of Pichia pastoris.

ABSTRACT:

Background

The effect of osmolarity on cellular physiology has been subject of investigation in many different species. High osmolarity is of importance for biotechnological production processes, where high cell densities and product titers are aspired. Several studies indicated that increased osmolarity of the growth medium can have a beneficial effect on recombinant protein production in different host organisms. Thus, the effect of osmolarity on the cellular physiology of Pichia pastoris, a prominent host for recombinant protein production, was studied in carbon limited chemostat cultures at different osmolarities. Transcriptome and proteome analyses were applied to assess differences upon growth at different osmolarities in both, a wild type strain and an antibody fragment expr

SUBMITTER: Dragosits M 

PROVIDER: S-EPMC2867824 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature