Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data.

ABSTRACT:

Background

Bacillus subtilis natto is closely related to the laboratory standard strain B. subtilis Marburg 168, and functions as a starter for the production of the traditional Japanese food "natto" made from soybeans. Although re-sequencing whole genomes of several laboratory domesticated B. subtilis 168 derivatives has already been attempted using short read sequencing data, the assembly of the whole genome sequence of a closely related strain, B. subtilis natto, from very short read data is more challenging, particularly with our aim to assemble one fully connected scaffold from short reads around 35 bp in length.

Results

We applied a comparative genome assembly method, which combines de novo assembly and reference guided assembly, to one of the B. subtilis natto strains

SUBMITTER: Nishito Y 

PROVIDER: S-EPMC2867830 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature