Quantitative analysis of intact apolipoproteins in human HDL by top-down differential mass spectrometry.

ABSTRACT: Top-down mass spectrometry holds tremendous potential for the characterization and quantification of intact proteins, including individual protein isoforms and specific posttranslationally modified forms. This technique does not require antibody reagents and thus offers a rapid path for assay development with increased specificity based on the amino acid sequence. Top-down MS is efficient whereby intact protein mass measurement, purification by mass separation, dissociation, and measurement of product ions with ppm mass accuracy occurs on the seconds to minutes time scale. Moreover, as the analysis is based on the accurate measurement of an intact protein, top-down mass spectrometry opens a research paradigm to perform quantitative analysis of "unknown" proteins that differ in accurate mas

SUBMITTER: Mazur MT 

PROVIDER: S-EPMC2867874 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature