Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys.

ABSTRACT:

Background

The highly pathogenic H5N1 is a major avian pathogen that crosses species barriers and seriously affects humans as well as some mammals. It mutates in an intensified manner and is considered a potential candidate for the possible next pandemic with all the catastrophic consequences.

Methods

Nasal swabs were collected from donkeys suffered from respiratory distress. The virus was isolated from the pooled nasal swabs in specific pathogen free embryonated chicken eggs (SPF-ECE). Reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing of both haemagglutingin and neuraminidase were performed. H5 seroconversion was screened using haemagglutination inhibition (HI) assay on 105 donkey serum samples.

Results

We demonstrated that H5N1 jumped from poul

SUBMITTER: Abdel-Moneim AS 

PROVIDER: S-EPMC2867947 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature