Cell wall proteome analysis of Mycobacterium smegmatis strain MC2 155.

ABSTRACT:

Background

The usually non-pathogenic soil bacterium Mycobacterium smegmatis is commonly used as a model mycobacterial organism because it is fast growing and shares many features with pathogenic mycobacteria. Proteomic studies of M. smegmatis can shed light on mechanisms of mycobacterial growth, complex lipid metabolism, interactions with the bacterial environment and provide a tractable system for antimycobacterial drug development. The cell wall proteins are particularly interesting in this respect. The aim of this study was to construct a reference protein map for these proteins in M. smegmatis.

Results

A proteomic analysis approach, based on one dimensional polyacrylamide gel electrophoresis and LC-MS/MS, was used to identify and characterize the cell wall associated pr

SUBMITTER: He Z 

PROVIDER: S-EPMC2867950 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature