NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer.

ABSTRACT:

Background

Nasopharyngeal carcinoma associated gene 6 (NGX6) is down-regulated in most colon cancer cell lines and tumor tissues when compared with their normal tissue samples. As a novel suppress tumor gene, it could inhibit colon cancer cell growth and cell cycle progression. However, little is known about the transcriptional mechanisms controlling NGX6 gene expression. Recent findings suggest that epigenetic inactivation of multiple tumor suppressor genes plays an important role in the tumorigenesis of colorectal carcinoma (CRC). In this study, we explored the role of DNA methylation in regulation of NGX6 transcription.

Methods

In the present study, we cloned the NGX6 promoter with characteristics of a CpG island by luciferase reporter assay. Then, the CpG methylation sta

SUBMITTER: Liu M 

PROVIDER: S-EPMC2868053 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature