Integrated stoichiometric, thermodynamic and kinetic modelling of steady state metabolism.

ABSTRACT: The quantitative analysis of biochemical reactions and metabolites is at frontier of biological sciences. The recent availability of high-throughput technology data sets in biology has paved the way for new modelling approaches at various levels of complexity including the metabolome of a cell or an organism. Understanding the metabolism of a single cell and multi-cell organism will provide the knowledge for the rational design of growth conditions to produce commercially valuable reagents in biotechnology. Here, we demonstrate how equations representing steady state mass conservation, energy conservation, the second law of thermodynamics, and reversible enzyme kinetics can be formulated as a single system of linear equalities and inequalities, in addition to linear equalities on exponenti

SUBMITTER: Fleming RM 

PROVIDER: S-EPMC2868105 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature