Apoptotic activity of caged xanthones from Garcinia hanburyi in cholangiocarcinoma cell lines.

ABSTRACT:

Aim

To investigate the growth inhibitory mechanism of four caged xanthones from Garcinia hanburyi in cholangiocarcinoma (CCA) KKU-100 and KKU-M156 cells.

Methods

Four caged xanthones, selected on the basis of their anticancer potency and chemical structure diversities (i.e. isomorellin, isomorellinol, forbesione and gambogic acid) were used in this study. Growth inhibition of these caged xanthones was determined using the sulforhodamine B assay. Induction of apoptosis was assessed by observing cell morphology, ethidium bromide and acridine orange staining and DNA fragmentation assay. Levels of apoptotic-related gene and protein expressions were determined by a real-time reverse transcriptase polymerase chain reaction and Western blotting analysis, respectively.

Results

SUBMITTER: Hahnvajanawong C 

PROVIDER: S-EPMC2868216 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature