Co-localization of the amyloid precursor protein and Notch intracellular domains in nuclear transcription factories.

ABSTRACT: The beta-amyloid precursor protein (APP) plays a major role in Alzheimer's disease. The APP intracellular domain (AICD), together with Fe65 and Tip60, localizes to spherical nuclear AFT complexes, which may represent sites of transcription. Despite a lack of co-localization with several described nuclear compartments, we have identified a close apposition between AFT complexes and splicing speckles, Cajal bodies and PML bodies. Live imaging revealed that AFT complexes were highly mobile within nuclei and following pharmacological inhibition of transcription fused into larger assemblies. We have previously shown that AICD regulates the expression of its own precursor APP. In support of our earlier findings, transfection of APP promoter plasmids as substrates resulted in cytosolic AFT comple

SUBMITTER: Konietzko U 

PROVIDER: S-EPMC2868363 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature