Posttranslational modifications of the bovine lens beaded filament proteins filensin and CP49.

ABSTRACT:

Purpose

The lens beaded filament proteins filensin and CP49 are phosphorylated proteins that undergo proteolytic degradation with fiber cell age; however, the specific sites of modifications remain largely unknown. The purpose of this study was to identify posttranslational modifications (PTMs) in bovine lens beaded filament proteins.

Methods

Filensin and CP49 were enriched by urea extraction of lens fiber cell homogenates after the water-soluble fraction was removed. The urea-soluble fraction was separated by SDS-PAGE, and the corresponding filensin and CP49 bands were digested by trypsin, Lys C, or Glu C. The enzymatic digests were analyzed by HPLC mass spectrometry.

Results

The sequences of lens beaded filament proteins were systematically mapped, and putative data

SUBMITTER: Wang Z 

PROVIDER: S-EPMC2868428 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature