Muscarinic acetylcholine receptor localization and activation effects on ganglion response properties.

ABSTRACT:

Purpose

The activation and blockade of muscarinic acetylcholine receptors (mAChRs) affects retinal ganglion cell light responses and firing rates. This study was undertaken to identify the full complement of mAChRs expressed in the rabbit retina and to assess mAChR distribution and the functional effects of mAChR activation and blockade on retinal response properties.

Methods

RT-PCR, Western blot analysis, and immunohistochemistry were used to identify the complement and distribution of mAChRs in the rabbit retina. Extracellular electrophysiology was used to determine the effects of the activation or blockade of mAChRs on ganglion cell response properties.

Results

RT-PCR of whole neural retina resulted in the amplification of mRNA transcripts for the m1 to m5 mAChR su

SUBMITTER: Strang CE 

PROVIDER: S-EPMC2868487 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature