Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains.

ABSTRACT:

Background

Activation of fatty acids by acyl-CoA synthetase enzymes is required for de novo lipid synthesis, fatty acid catabolism, and remodeling of biological membranes. Human long-chain acyl-CoA synthetase member 6, ASCL6, is a form present in the plasma membrane of cells. Splicing events affecting the amino-terminus and alternative motifs near the ATP-binding site generate different isoforms of ACSL6.

Results

Isoforms with different fatty acid Gate-domain motifs have different activity and the form lacking this domain, isoform 3, showed no detectable activity. Enzymes truncated of the first 40 residues generate acyl-CoAs at a faster rate than the full-length protein. The gating residue, which prevents entry of the fatty acid substrate unless one molecule of ATP has alrea

SUBMITTER: Soupene E 

PROVIDER: S-EPMC2868784 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature