Roles of AP-2 in clathrin-mediated endocytosis.

ABSTRACT:

Background

The notion that AP-2 clathrin adaptor is an essential component of an endocytic clathrin coat appears to conflict with recent observations that substantial AP-2 depletion, using RNA interference with synthesis of AP-2 subunits, fails to block uptake of certain ligands known to internalize through a clathrin-based pathway.

Methodology/principal findings

We report here the use of in vivo imaging data obtained by spinning-disk confocal microscopy to study the formation of clathrin-coated structures at the plasma membranes of BSC1 and HeLa cells depleted by RNAi of the clathrin adaptor, AP-2. Very few clathrin coats continue to assemble after AP-2 knockdown. Moreover, there is a total absence of clathrin-containing structures completely lacking AP-2 while all the rema

SUBMITTER: Boucrot E 

PROVIDER: S-EPMC2868873 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature