Multiple promoters and alternative splicing: Hoxa5 transcriptional complexity in the mouse embryo.

ABSTRACT:

Background

The genomic organization of Hox clusters is fundamental for the precise spatio-temporal regulation and the function of each Hox gene, and hence for correct embryo patterning. Multiple overlapping transcriptional units exist at the Hoxa5 locus reflecting the complexity of Hox clustering: a major form of 1.8 kb corresponding to the two characterized exons of the gene and polyadenylated RNA species of 5.0, 9.5 and 11.0 kb. This transcriptional intricacy raises the question of the involvement of the larger transcripts in Hox function and regulation.

Methodology/principal findings

We have undertaken the molecular characterization of the Hoxa5 larger transcripts. They initiate from two highly conserved distal promoters, one corresponding to the putative Hoxa6 promoter,

SUBMITTER: Coulombe Y 

PROVIDER: S-EPMC2868907 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature