Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis.

ABSTRACT: The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

SUBMITTER: Sazhenskiy V 

PROVIDER: S-EPMC2869120 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature