Cardiac stem cell genetic engineering using the alphaMHC promoter.

ABSTRACT:

Aims

Cardiac stem cells (CSCs) show potential as a cellular therapeutic approach to blunt tissue damage and facilitate reparative and regenerative processes after myocardial infarction. Despite multiple published reports of improvement, functional benefits remain modest using normal stem cells delivered by adoptive transfer into damaged myocardium. The goal of this study is to enhance survival and proliferation of CSCs that have undergone lineage commitment in early phases as evidenced by expression of proteins driven by the alpha-myosin heavy chain (alphaMHC) promoter. The early increased expression of survival kinases augments expansion of the cardiogenic CSC pool and subsequent daughter progeny.

Materials & methods

Normal CSCs engineered with fluorescent reporter protein

SUBMITTER: Bailey B 

PROVIDER: S-EPMC2869202 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature