Proper organization of microtubule minus ends is needed for midzone stability and cytokinesis.

ABSTRACT: Successful cytokinesis is critical for maintaining genome stability and requires the assembly of a robust central spindle to specify the cleavage furrow position, to prevent separated chromosomes from coming back together, and to contribute to midbody abscission. A proper central spindle is assembled and maintained by a number of microtubule-associated and molecular motor proteins that sort microtubules into bundles with their plus ends overlapping at the center. The mechanisms by which different factors organize the central spindle microtubules remain unclear. We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequency of polyploid cells, which resulted from a failure in cytokinesis. In addition, HSET knockdown resulted in severe midzone microtubule organi

SUBMITTER: Cai S 

PROVIDER: S-EPMC2869383 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature