Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032.

ABSTRACT:

Background

MFN1032 is a clinical Pseudomonas fluorescens strain able to grow at 37 degrees C. MFN1032 cells induce necrosis and apoptosis in rat glial cells at this temperature. This strain displays secretion-mediated hemolytic activity involving phospholipase C and cyclolipopeptides. Under laboratory conditions, this activity is not expressed at 37 degrees C. This activity is tightly regulated and is subject to phase variation.

Results

We found that MFN1032 displays a cell-associated hemolytic activity distinct from the secreted hemolytic activity. Cell-associated hemolysis was expressed at 37 degrees C and was only detected in vitro in mid log growth phase in the presence of erythrocytes. We studied the regulation of this activity in the wild-type strain and in a mutant de

SUBMITTER: Sperandio D 

PROVIDER: S-EPMC2871272 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature