A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency.

ABSTRACT: Protein complexes and protein-protein interactions are essential for almost all cellular processes. Here, we establish a mammalian affinity purification and lentiviral expression (MAPLE) system for characterizing the subunit compositions of protein complexes. The system is flexible (i.e. multiple N- and C-terminal tags and multiple promoters), is compatible with Gateway cloning, and incorporates a reference peptide. Its major advantage is that it permits efficient and stable delivery of affinity-tagged open reading frames into most mammalian cell types. We benchmarked MAPLE with a number of human protein complexes involved in transcription, including the RNA polymerase II-associated factor, negative elongation factor, positive transcription elongation factor b, SWI/SNF, and mixed lineage l

SUBMITTER: Mak AB 

PROVIDER: S-EPMC2871416 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature