A statistics-based platform for quantitative N-terminome analysis and identification of protease cleavage products.

ABSTRACT: Terminal amine isotopic labeling of substrates (TAILS), our recently introduced platform for quantitative N-terminome analysis, enables wide dynamic range identification of original mature protein N-termini and protease cleavage products. Modifying TAILS by use of isobaric tag for relative and absolute quantification (iTRAQ)-like labels for quantification together with a robust statistical classifier derived from experimental protease cleavage data, we report reliable and statistically valid identification of proteolytic events in complex biological systems in MS2 mode. The statistical classifier is supported by a novel parameter evaluating ion intensity-dependent quantification confidences of single peptide quantifications, the quantification confidence factor (QCF). Furthermore, the isof

SUBMITTER: auf dem Keller U 

PROVIDER: S-EPMC2871423 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature