Axonal transport proteomics reveals mobilization of translation machinery to the lesion site in injured sciatic nerve.

ABSTRACT: Investigations of the molecular mechanisms underlying responses to nerve injury have highlighted the importance of axonal transport systems. To obtain a comprehensive view of the protein ensembles associated with axonal transport in injured axons, we analyzed the protein compositions of axoplasm concentrated at ligatures following crush injury of rat sciatic nerve. LC-MS/MS analyses of iTRAQ-labeled peptides from axoplasm distal and proximal to the ligation sites revealed protein ensembles transported in both anterograde and retrograde directions. Variability of replicates did not allow straightforward assignment of proteins to functional transport categories; hence, we performed principal component analysis and factor analysis with subsequent clustering to determine the most prominent inj

SUBMITTER: Michaelevski I 

PROVIDER: S-EPMC2871428 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature