Ontology highlight
ABSTRACT:
SUBMITTER: Brandao TA
PROVIDER: S-EPMC2871455 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Brandão Tiago A S TA Hengge Alvan C AC Johnson Sean J SJ
The Journal of biological chemistry 20100316 21
Catalysis by protein-tyrosine phosphatase 1B (PTP1B) occurs through a two-step mechanism involving a phosphocysteine intermediate. We have solved crystal structures for the transition state analogs for both steps. Together with previously reported crystal structures of apo-PTP1B, the Michaelis complex of an inactive mutant, the phosphoenzyme intermediate, and the product complex, a full picture of all catalytic steps can now be depicted. The transition state analog for the first catalytic step c ...[more]