Diverse modes of Drosophila tracheal fusion cell transcriptional regulation.

ABSTRACT: Drosophila tracheal fusion cells play multiple important roles in guiding and facilitating tracheal branch fusion. Mechanistic understanding of how fusion cells function during development requires deciphering their transcriptional circuitry. In this paper, three genes with distinct patterns of fusion cell expression were dissected by transgenic analysis to identify the cis-regulatory modules that mediate their transcription. Bioinformatic analysis involving phylogenetic comparisons coupled with mutational experiments were employed. The dysfusion bHLH-PAS gene was shown to have two fusion cell cis-regulatory modules; one driving initial expression and another autoregulatory module to enhance later transcription. Mutational dissection of the early module identified at least four distinct in

SUBMITTER: Jiang L 

PROVIDER: S-EPMC2872039 | biostudies-literature | 2010 May-Jun

REPOSITORIES: biostudies-literature