Key roles for MED1 LxxLL motifs in pubertal mammary gland development and luminal-cell differentiation.

ABSTRACT: Mediator recently has emerged as a central player in the direct transduction of signals from transcription factors to the general transcriptional machinery. In the case of nuclear receptors, in vitro studies have shown that the transcriptional coactivator function of the Mediator involves direct ligand-dependent interactions of the MED1 subunit, through its two classical LxxLL motifs, with the receptor AF2 domain. However, despite the strong in vitro evidence, there currently is little information regarding in vivo functions of the LxxLL motifs either in MED1 or in other coactivators. Toward this end, we have generated MED1 LxxLL motif-mutant knockin mice. Interestingly, these mice are both viable and fertile and do not exhibit any apparent gross abnormalities. However, they do exhibit sev

SUBMITTER: Jiang P 

PROVIDER: S-EPMC2872411 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature