Large-scale analysis of DNA methylation in chronic lymphocytic leukemia.

ABSTRACT:

Aims

B-cell chronic lymphocytic leukemia (CLL) is a heterogeneous malignancy that clinically ranges from indolent to rapidly progressive. CLL, like other cancers, can be affected by epigenetic alterations.

Materials & methods

A microarray discovery-based study was initiated to determine DNA methylation in CLL cases with a range of CD38 expression (1–92%).

Results

Many loci were either methylated or unmethylated across all CD38 levels, but differential methylation was also observed for some genes. Genomic sequencing of DLEU7 confirmed extensive cytosine methylation preferentially in patient samples with low CD38 expression, whereas NRP2, SFRP2 and ADAM12 were more commonly methylated in those with high CD38 expression.

Conclusion

This study demonstrates that CLL

SUBMITTER: Rahmatpanah FB 

PROVIDER: S-EPMC2872502 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature