Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions.

ABSTRACT: Protein interactions are involved in all cellular processes. Their efficient and reliable characterization is therefore essential for understanding biological mechanisms. In this study, we show that combining bacterial artificial chromosome (BAC) TransgeneOmics with quantitative interaction proteomics, which we call quantitative BAC-green fluorescent protein interactomics (QUBIC), allows specific and highly sensitive detection of interactions using rapid, generic, and quantitative procedures with minimal material. We applied this approach to identify known and novel components of well-studied complexes such as the anaphase-promoting complex. Furthermore, we demonstrate second generation interaction proteomics by incorporating directed mutational transgene modification and drug perturbation

SUBMITTER: Hubner NC 

PROVIDER: S-EPMC2872919 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature