Computer controlled automated assay for comprehensive studies of enzyme kinetic parameters.

ABSTRACT: Stability and biological activity of proteins is highly dependent on their physicochemical environment. The development of realistic models of biological systems necessitates quantitative information on the response to changes of external conditions like pH, salinity and concentrations of substrates and allosteric modulators. Changes in just a few variable parameters rapidly lead to large numbers of experimental conditions, which go beyond the experimental capacity of most research groups. We implemented a computer-aided experimenting framework ("robot lab assistant") that allows us to parameterize abstract, human-readable descriptions of micro-plate based experiments with variable parameters and execute them on a conventional 8 channel liquid handling robot fitted with a sensitive plate r

SUBMITTER: Bonowski F 

PROVIDER: S-EPMC2873295 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature