Characterization of recombinant beta-fructofuranosidase from Bifidobacterium adolescentis G1.

ABSTRACT:

Background

We have previously reported on purification and characterization of beta-fructofuranosidase (beta-FFase) from Bifidobacterium adolescentis G1. This enzyme showed high activity of hydrolysis on fructo-oligosaccharides with a low degree of polymerization. Recently, genome sequences of B. longum NCC2705 and B. adolescentis ATCC 15703 were determined, and cscA gene in the both genome sequences encoding beta-FFase was predicted. Here, cloning of cscA gene encoding putative beta-FFase from B. adolescentis G1, its expression in E. coli and properties of the recombinant protein are described.

Results

Using the information of cscA gene from Bifidobacterium adolescentis ATCC 15703, cscA gene from B. adolescentis G1 was cloned and sequenced. The N-terminal amino acid sequenc

SUBMITTER: Omori T 

PROVIDER: S-EPMC2873357 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature