Oxidative stress in NSC-741909-induced apoptosis of cancer cells.

ABSTRACT:

Background

NSC-741909 is a novel anticancer agent that can effectively suppress the growth of several cell lines derived from lung, colon, breast, ovarian, and kidney cancers. We recently showed that NSC-741909-induced antitumor activity is associated with sustained Jun N-terminal kinase (JNK) activation, resulting from suppression of JNK dephosphorylation associated with decreased protein levels of MAPK phosphatase-1. However, the mechanisms of NSC-741909-induced antitumor activity remain unclear. Because JNK is frequently activated by oxidative stress in cells, we hypothesized that reactive oxygen species (ROS) may be involved in the suppression of JNK dephosphorylation and the cytotoxicity of NSC-741909.

Methods

The generation of ROS was measured by using the cell-permeab

SUBMITTER: Wei X 

PROVIDER: S-EPMC2873373 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature