Discovery of high-affinity protein binding ligands--backwards.

ABSTRACT:

Background

There is a pressing need for high-affinity protein binding ligands for all proteins in the human and other proteomes. Numerous groups are working to develop protein binding ligands but most approaches develop ligands using the same strategy in which a large library of structured ligands is screened against a protein target to identify a high-affinity ligand for the target. While this methodology generates high-affinity ligands for the target, it is generally an iterative process that can be difficult to adapt for the generation of ligands for large numbers of proteins.

Methodology/principal findings

We have developed a class of peptide-based protein ligands, called synbodies, which allow this process to be run backwards--i.e. make a synbody and then screen it agai

SUBMITTER: Diehnelt CW 

PROVIDER: S-EPMC2873402 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature