Identification of miRNA from Porphyra yezoensis by high-throughput sequencing and bioinformatics analysis.

ABSTRACT:

Background

miRNAs are a class of non-coding, small RNAs that are approximately 22 nucleotides long and play important roles in the translational level regulation of gene expression by either directly binding or cleaving target mRNAs. The red alga, Porphyra yezoensis is one of the most important marine economic crops worldwide. To date, only a few miRNAs have been identified in green unicellar alga and there is no report about Porphyra miRNAs.

Methodology/principal findings

To identify miRNAs in Porphyra yezoensis, a small RNA library was constructed. Solexa technology was used to perform high throughput sequencing of the library and subsequent bioinformatics analysis to identify novel miRNAs. Specifically, 180,557,942 reads produced 13,324 unique miRNAs representing 224 cons

SUBMITTER: Liang C 

PROVIDER: S-EPMC2873431 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature