'Loss of pigment epithelium-derived factor enables migration, invasion and metastatic spread of human melanoma'.

ABSTRACT: Pigment epithelium-derived factor (PEDF) is a multifunctional secreted glycoprotein that displays broad anti-tumor activity based on dual targeting of the tumor microenvironment (anti-angiogenic action) and the tumor cells (direct anti-tumor action). Here, we show that PEDF expression is high in melanocytes, but it is lost during malignant progression of human melanoma. Using a high-throughput analysis of the data from microarray studies of molecular profiling of human melanoma, we found that PEDF expression is lost in highly invasive melanomas. In paired cell lines established from the same lesion but representing the high and low extremes of malignant potential, abundant PEDF expression was restricted to the poorly aggressive counterparts. We used RNA interference to directly address the

SUBMITTER: Orgaz JL 

PROVIDER: S-EPMC2873770 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature