Discrimination of thermophilic and mesophilic proteins.

ABSTRACT:

Background

There is a considerable literature on the source of the thermostability of proteins from thermophilic organisms. Understanding the mechanisms for this thermostability would provide insights into proteins generally and permit the design of synthetic hyperstable biocatalysts.

Results

We have systematically tested a large number of sequence and structure derived quantities for their ability to discriminate thermostable proteins from their non-thermostable orthologs using sets of mesophile-thermophile ortholog pairs. Most of the quantities tested correspond to properties previously reported to be associated with thermostability. Many of the structure related properties were derived from the Delaunay tessellation of protein structures.

Conclusions

Carefully sele

SUBMITTER: Taylor TJ 

PROVIDER: S-EPMC2873828 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature