Baculoviral capsid display of His-tagged ZnO inorganic binding peptide.

ABSTRACT: Virus-templated fabrication of compound structures can be made through incorporating the specifically inorganic-binding peptide into the viral scaffold, widely used is phage display system. Compared to prokaryotic phages, insect cell-based baculovirus has some strengths such as the adaptability to the proteins' posttranslational modification and non-replication in mammalian cells. As an attempt to explore the baculovirus-mediated bioconjugates, we show in this study that a genetically engineered baculovirus, with a hexahistidine (His(6)) tagged ZnO binding peptide fused to the N-terminus of the viral capsid protein vp39 of AcNPV, was constructed. It maintains both the viral infectivity and the fusion protein's activity. The presence of the fusion protein on the baculovirus particle was dem

SUBMITTER: Song L 

PROVIDER: S-EPMC2873982 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature