Dataset Information
Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines.
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ABSTRACT: Aim
To investigate the effects of butyrate on interleukin (IL)-32alpha expression in epithelial cell lines.Methods
The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32alpha was determined by Western blotting analyses. IL-32alpha mRNA expression was analyzed by real-time polymerase chain reaction.Results
Acetate and propionate had no effects on IL-32alpha mRNA expression. Butyrate significantly enhanced IL-32alpha expression in all cell lines. Butyrate also up-regulated IL-1beta-induced IL-32alpha mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL-32alpha expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1beta-induced IL-32alpha mRNA expression.Conclusion
Butyrate stimulated IL-32alpha expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32alpha expression.
SUBMITTER: Kobori A
PROVIDER: S-EPMC2874139 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Publications
Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines.
World journal of gastroenterology 20100501 19
<h4>Aim</h4>To investigate the effects of butyrate on interleukin (IL)-32alpha expression in epithelial cell lines.<h4>Methods</h4>The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32alpha was determined by Western blotting analyses. IL-32alpha mRNA expression was analyzed by real-time polymerase chain reaction.<h4>Results</h4>Acetate and propionate had no effects on IL-32alpha mRNA expression. Butyrate significantly enhanced IL-32alpha expression in al ...[more]