Differential transcript expression between the microfilariae of the filarial nematodes, Brugia malayi and B. pahangi.

ABSTRACT:

Background

Brugia malayi and B. pahangi are two closely related nematodes that cause filariasis in humans and animals. However, B. pahangi microfilariae are able to develop in and be transmitted by the mosquito, Armigeres subalbatus, whereas most B. malayi are rapidly melanized and destroyed within the mosquito hemocoel. A cross-species microarray analysis employing the B. malayi V2 array was carried out to determine the transcriptional differences between B. malayi and B. pahangi microfilariae with similar age distribution.

Results

Following microarray data analysis, a list of preferentially expressed genes in both microfilariae species was generated with a false discovery rate estimate of 5% and a signal intensity ratio of 2 or higher in either species. A total of 308 prob

SUBMITTER: Kariuki MM 

PROVIDER: S-EPMC2874553 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature