Local non-esterified fatty acids correlate with inflammation in atheroma plaques of patients with type 2 diabetes.

ABSTRACT:

Objective

Atherosclerosis is prevalent in diabetic patients, but there is little information on the localization of nonesterified fatty acids (NEFAs) within the plaque and their relationship with inflammation. We sought to characterize the NEFA composition and location in human diabetic atheroma plaques by metabolomic analysis and imaging and to address their relationship with inflammation activity.

Research design and methods

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) was used for metabolomic analysis imaging of frozen carotid atheroma plaques. Carotid endarterectomy specimens were used for conventional immunohistochemistry, laser-capture microdissection quantitative PCR, and in situ Southwestern hybridization. Biological actions of linoleic acid were studied

SUBMITTER: Mas S 

PROVIDER: S-EPMC2874689 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature