APIS: accurate prediction of hot spots in protein interfaces by combining protrusion index with solvent accessibility.

ABSTRACT:

Background

It is well known that most of the binding free energy of protein interaction is contributed by a few key hot spot residues. These residues are crucial for understanding the function of proteins and studying their interactions. Experimental hot spots detection methods such as alanine scanning mutagenesis are not applicable on a large scale since they are time consuming and expensive. Therefore, reliable and efficient computational methods for identifying hot spots are greatly desired and urgently required.

Results

In this work, we introduce an efficient approach that uses support vector machine (SVM) to predict hot spot residues in protein interfaces. We systematically investigate a wide variety of 62 features from a combination of protein sequence and structure in

SUBMITTER: Xia JF 

PROVIDER: S-EPMC2874803 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature