Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum.

ABSTRACT:

Background

The dinoflagellate Alexandrium minutum typically produces paralytic shellfish poisoning (PSP) toxins, which are known only from cyanobacteria and dinoflagellates. While a PSP toxin gene cluster has recently been characterized in cyanobacteria, the genetic background of PSP toxin production in dinoflagellates remains elusive.

Results

We constructed and analysed an expressed sequence tag (EST) library of A. minutum, which contained 15,703 read sequences yielding a total of 4,320 unique expressed clusters. Of these clusters, 72% combined the forward-and reverse reads of at least one bacterial clone. This sequence resource was then used to construct an oligonucleotide microarray. We analysed the expression of all clusters in three different strains. While the cyanobac

SUBMITTER: Yang I 

PROVIDER: S-EPMC2874808 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature