High-throughput marker discovery in melon using a self-designed oligo microarray.

ABSTRACT:

Background

Genetic maps constitute the basis of breeding programs for many agricultural organisms. The creation of these maps is dependent on marker discovery. Melon, among other crops, is still lagging in genomic resources, limiting the ability to discover new markers in a high-throughput fashion. One of the methods used to search for molecular markers is DNA hybridization to microarrays. Microarray hybridization of DNA from different accessions can reveal differences between them--single-feature polymorphisms (SFPs). These SFPs can be used as markers for breeding purposes, or they can be converted to conventional markers by sequencing. This method has been utilized in a few different plants to discover genetic variation, using Affymetrix arrays that exist for only a few organisms

SUBMITTER: Ophir R 

PROVIDER: S-EPMC2874814 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature