Array-based whole-genome survey of dog saliva DNA yields high quality SNP data.

ABSTRACT:

Background

Genome-wide association scans for genetic loci underlying both Mendelian and complex traits are increasingly common in canine genetics research. However, the demand for high-quality DNA for use on such platforms creates challenges for traditional blood sample ascertainment. Though the use of saliva as a means of collecting DNA is common in human studies, alternate means of DNA collection for canine research have instead been limited to buccal swabs, from which dog DNA is of insufficient quality and yield for use on most high-throughput array-based systems. We thus investigated an animal-based saliva collection method for ease of use and quality of DNA obtained and tested the performance of saliva-extracted canine DNA on genome-wide genotyping arrays.

Methodology/princ

SUBMITTER: Yokoyama JS 

PROVIDER: S-EPMC2876042 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature