Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane.

ABSTRACT:

Background

The plasma membrane (PM) is a compartment of significant interest because cell surface proteins influence the way in which a cell interacts with its neighbours and its extracellular environment. However, PM is hard to isolate because of its low abundance. Aqueous two-phase affinity purification (2PAP), based on PEG/Dextran two-phase fractionation and lectin affinity for PM-derived microsomes, is an emerging method for the isolation of high purity plasma membranes from several vertebrate sources. In contrast, PM isolation techniques in important invertebrate genetic model systems, such as Drosophila melanogaster, have relied upon enrichment by density gradient centrifugation. To facilitate genetic investigation of activities contributing to the content of the PM sub-prote

SUBMITTER: Khanna MR 

PROVIDER: S-EPMC2876126 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature