A critical assessment of cross-species detection of gene duplicates using comparative genomic hybridization.

ABSTRACT:

Background

Comparison of genomic DNA among closely related strains or species is a powerful approach for identifying variation in evolutionary processes. One potent source of genomic variation is gene duplication, which is prevalent among individuals and species. Array comparative genomic hybridization (aCGH) has been successfully utilized to detect this variation among lineages. Here, beyond the demonstration that gene duplicates among species can be quantified with aCGH, we consider the effect of sequence divergence on the ability to detect gene duplicates.

Results

Using the X chromosome genomic content difference between male D. melanogaster and female D. yakuba and D. simulans, we describe a decrease in the ability to accurately measure genomic content (copy number) for

SUBMITTER: Machado HE 

PROVIDER: S-EPMC2876127 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature