Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species.

ABSTRACT:

Background

Genotype analysis using multiple single nucleotide polymorphisms (SNPs) is a useful but labor-intensive or high-cost procedure in plant research. Here we describe an alternative genotyping method that is suited to multi-sample or multi-locus SNP genotyping and does not require electrophoresis or specialized equipment.

Results

We have developed a simple method for multi-sample or multi-locus SNP genotyping using allele-specific primers (ASP). More specifically, we (1) improved the design of allele-specific primers, (2) established a method to detect PCR products optically without electrophoresis, and (3) standardized PCR conditions for parallel genomic assay using various allele-specific primers. As an illustration of multi-sample SNP genotyping using ASP, we mappe

SUBMITTER: Hirotsu N 

PROVIDER: S-EPMC2876155 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature