Discovery of a gene conferring multiple-aminoglycoside resistance in Escherichia coli.

ABSTRACT: Bovine-origin Escherichia coli isolates were tested for resistance phenotypes using a disk diffusion assay and for resistance genotypes using a DNA microarray. An isolate with gentamicin and amikacin resistance but with no corresponding genes detected yielded a 1,056-bp DNA sequence with the closest homologues for its inferred protein sequence among a family of 16S rRNA methyltransferase enzymes. These enzymes confer high-level aminoglycoside resistance and have only recently been described in Gram-negative bacteria.

SUBMITTER: Davis MA 

PROVIDER: S-EPMC2876372 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature