CXCL4 downregulates the atheroprotective hemoglobin receptor CD163 in human macrophages.

ABSTRACT:

Rationale

CXCL4 is a platelet-derived chemokine that promotes macrophage differentiation from monocytes. Deletion of the PF4 gene that encodes CXCL4 reduces atherosclerotic lesions in ApoE(-/-) mice.

Objective

We sought to study effects of CXCL4 on macrophage differentiation with possible relevance for atherogenesis.

Methods and results

Flow cytometry for expression of surface markers in macrophage colony-stimulating factor (M-CSF)- and CXCL4-induced macrophages demonstrated virtually complete absence of the hemoglobin scavenger receptor CD163 in CXCL4-induced macrophages. mRNA for CD163 was downregulated as early as 2 hours after CXCL4. CD163 protein reached a minimum after 3 days, which was not reversed by treatment of cells with M-CSF. The CXCL4 effect was entirely

SUBMITTER: Gleissner CA 

PROVIDER: S-EPMC2876722 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature