Expression of human eukaryotic initiation factor 3f oscillates with cell cycle in A549 cells and is essential for cell viability.

ABSTRACT:

Background

Transcriptional and postranslational regulation of the cell cycle has been widely studied. However, there is scarce knowledge concerning translational control of this process. Several mammalian eukaryotic initiation factors (eIFs) seem to be implicated in controlling cell proliferation. In this work, we investigated if the human eIF3f expression and function is cell cycle related.

Results

The human eIF3f expression has been found to be upregulated in growth-stimulated A549 cells and downregulated in G0. Western blot analysis and eIF3f promotor-luciferase fusions revealed that eIF3f expression peaks twice in the cell cycle: in the S and the M phases. Deregulation of eIF3f expression negatively affects cell viability and induces apoptosis.

Conclusions

The exp

SUBMITTER: Higareda-Mendoza AE 

PROVIDER: S-EPMC2877012 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature